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Baboons infected with human immunodeficiency virus type 1 (HIV-1) have been used as an animal model to study virus-host immune interactions. In this study, the polypeptide-specific immune response induced by HIV-1 antigens is analyzed in the spleen and lymph nodes of African green monkeys in vivo as well as in the spleen and Peyer's patch of ex vivo, cell-cultured baboons. Expression of virus structural and nonstructural proteins in virus-infected T cells and macrophages was determined by immunocytochemistry. In addition, the HIV-1 polypeptide-specific cell-mediated cytotoxic response was analyzed in vivo by the use of labeled target cells. The study provides evidence that baboons infected with HIV-1 were able to produce HIV-1 antigen-specific humoral and cell-mediated immune responses. 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